| Title | RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling. |
| Publication Type | Journal Article |
| Year of Publication | 2021 |
| Authors | Jeancolas C, Matsubara YJ, Vybornyi M, Lambert CN, Blokhuis A, Alline T, Griffiths AD, Ameta S, Krishna S, Nghe P |
| Journal | Chem Commun (Camb) |
| Volume | 57 |
| Issue | 61 |
| Pagination | 7517-7520 |
| Date Published | 2021 Jul 29 |
| ISSN | 1364-548X |
| Keywords | Azoarcus, Biocatalysis, Genetic Phenomena, High-Throughput Nucleotide Sequencing, Inverted Repeat Sequences, Kinetics, Recombinases, RNA, RNA, Bacterial, RNA, Catalytic |
| Abstract | We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks. |
| DOI | 10.1039/d1cc02290c |
| Alternate Journal | Chem Commun (Camb) |
| PubMed ID | 34235521 |
| PubMed Central ID | PMC8320737 |
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