|Title||Exploiting cell-to-cell variability to detect cellular perturbations.|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Dey G, Gupta GD, Ramalingam B, Sathe M, Mayor S, Thattai M|
|Keywords||Animals, Cell Shape, Drosophila, Endocytosis, Phenotype, RNA Interference, RNA, Small Interfering|
Any single-cell-resolved measurement generates a population distribution of phenotypes, characterized by a mean, a variance, and a shape. Here we show that changes in the shape of a phenotypic distribution can signal perturbations to cellular processes, providing a way to screen for underlying molecular machinery. We analyzed images of a Drosophila S2R+ cell line perturbed by RNA interference, and tracked 27 single-cell features which report on endocytic activity, and cell and nuclear morphology. In replicate measurements feature distributions had erratic means and variances, but reproducible shapes; RNAi down-regulation reliably induced shape deviations in at least one feature for 1072 out of 7131 genes surveyed, as revealed by a Kolmogorov-Smirnov-like statistic. We were able to use these shape deviations to identify a spectrum of genes that influenced cell morphology, nuclear morphology, and multiple pathways of endocytosis. By preserving single-cell data, our method was even able to detect effects invisible to a population-averaged analysis. These results demonstrate that cell-to-cell variability contains accessible and useful biological information, which can be exploited in existing cell-based assays.
|Alternate Journal||PLoS ONE|
|PubMed Central ID||PMC3942435|